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Colorado (9 DIABETES.JPG) .UGANDA (20070918.jpeg) .FEBRUARY (20171201.JPG)The invention relates to a method for treating a substrate comprising immobilising at least a biomolecule on at least a part of the substrate, detecting free, i.e. unbound and unbound as a result of detection, biomolecule, and optionally delivering the biologically active biomolecule. Said substrates may be test samples. The invention also relates to a biochip comprising a support, at least a biomolecule and a means of detecting a free, i.e. unbound biomolecule present on the substrate. So far, known methods for detecting a biomolecule on a substrate comprise detecting a hybridisation event between a target biomolecule and a probe, for example by detecting the formation of a duplex between a nucleic acid target sequence and a nucleic acid probe or a probe and its target (WO 02/069653). Other detection methods have been described for example by Ford in “A biologically relevant optical sensor based on the hybridization of a fluorescent oligonucleotide probe with surface immobilized DNA” (Biotechnology and Bioengineering Vol. 95, No. 1, 2002). Also known are methods for treatment of a substrate comprising immobilising at least a biomolecule on at least a part of the substrate, detecting free, i.e. unbound and unbound as a result of detection, biomolecule, and optionally delivering the biologically active biomolecule to the substrate. It is known to treat substrates by attaching a nucleic acid on its surface. US 2007/0204242 describes a nucleic acid hybridisation assay method for detecting and quantifying a nucleic acid of interest on or in a sample. The method is characterised in that a nucleic acid on the surface of a biosensor is contacted with a test sample comprising a nucleic acid of interest. The nucleic acid of interest may be attached to a naturally occurring nucleic acid, such as a DNA or a RNA, as a result of, for example, a hybridisation event. In a next step, the presence of the nucleic acid of interest is detected by detecting a subsequent hybridisation event. U.S. Pat. No. 5,744,311 describes a method for detection and/or quantification of analytes, especially nucle c6a93da74d

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