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In a subsequent study, PFTs were performed on a larger sample of samples, but PFTs did not distinguish between the degree of neuromuscular weakness. This is because many PFTs, such as the 10-metre walk, are influenced by physical impairments that make their interpretation uncertain, and others are of little use in Duchenne syndrome [26].

Concurrent validity of the physical functioning tests was generally acceptable although the grip strength test in general and triceps strength test only in boys had low to modest associations. The results did not change after those with missing or outlying values were removed. However, test-retest reliability was moderate to good for most tests.

To enhance an automated method to determine the ApP that replicates the same amount of TD, HIRD, TSD, Acc/Dec (normalized as meters covered in one minute) and Pmet (normalized as W·kg-1) throughout all official matches, a linear regression analysis was conducted between the recorded ApPs and the game demands. In addition to ball possession (MSL), the ApPs in scoring ([BA], [PS] and [WS]), dribbling ([AD] and [SD]), shooting ([S] and [C]), and passing ([C] and [PR]) were analyzed. The ratio of the number of individual movements (such as ball possession, dribbling, passing, and scoring) to the total number of’real’ minutes were calculated to identify the relative importance of individual movements. The coefficient of determination (R2) of these linear regressions were 0.65, 0.54, 0.47, 0.48, 0.57, 0.50, 0.40, 0.41, 0.38, 0.39, and 0.35, respectively. These regression analyses indicated a strong to excellent positive relationship between the recorded ApPs and the recorded TD, HIRD, TSD, Acc/Dec (normalized as meters covered in one minute) and Pmet (normalized as W·kg-1) during all official matches.

The testes of the male subjects were ranked from left to right and no distinction was made on whether the right or left side of the body was dominant. In addition, the groove was similar to the adductor major (left side), which was confidently identified by the subjects. The parameters of the testes were measured using a ruler for three points on the groin [22].

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