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… Asa.[Quantitative analytical method of saponins using capillary electrophoresis with indirect photometric detection].
To establish a simple and sensitive capillary electrophoresis (CE) method for the analysis of saponins. The separation was performed on the basis of a buffer containing 50 mmol.L(-1) borax and 100 mmol.L(-1)sodium phosphate, run at a flow rate of 0.7 cm.min(-1) and at a voltage of 15 kV. The separation was conducted with indirect detection by measuring the absorbance of 5 mum phthalate. The separation time was 13 min and the injection of sample was made into an approximately 1.0 cm long 25 mum i.d. capillary. The samples were spiked with the internal standard saikosaponin-c as the test sample. The calibration curves were linear with R(2) values from 0.9998 to 0.9999 and the detection limit was 3 mumol.L(-1) for ginsenoside Rd, and 2 mumol.L(-1) for saikosaponin-c. The recoveries were 100.3% -103.5% for ginsenoside Rd and 102.6% -107.4% for saikosaponin-c with their relative standard deviations of 0.48% -0.60% (n = 7). The proposed CE method is a simple and reproducible method for the quantitative analysis of saponins in ginseng.Vladimir Jablanović

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