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The therapeutic potential of cells administered in vivo is most likely restricted by their low survival rates. While mesenchymal stem cells (MSCs) are highly sensitive to hypoxia, conditioned medium from hypoxic cells increases the survival of MSCs in vitro. We have previously shown that human MSCs (hMSCs) in vitro respond to hypoxia or conditioned media from hypoxic cells by a change in morphology to a spindle-shaped phenotype and an increased expression of HIF1-alpha protein \[[@CR1]\]. This suggests that the hypoxia-associated phenotype is induced in vitro by soluble factors secreted by hypoxic hMSCs. In this study we investigate if the hypoxia-induced phenotype can be induced in vitro in undifferentiated hMSCs by conditioned medium of hypoxic hMSCs.

Methods {#Sec2}
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Hypoxic hMSCs were established by two-step hypoxic preconditioning of hMSCs. For these experiments, hMSCs were derived from placental tissue of uncomplicated, term pregnancies and expanded in culture. Hypoxic hMSC-conditioned media were obtained from culture medium of hypoxic hMSCs cultivated for 48 hours. Culture medium was carefully removed and replaced with an equal volume of fresh medium. Culture medium from normoxic cells was collected as control. hMSCs were incubated with hypoxic or normoxic conditioned media for 4 hours, 6 hours or 24 hours. hMSCs were then fixed and immunostained for HIF1-alpha, HIF2-alpha and HIF3-alpha. hMSCs were also stained with propidium iodide for analysis of cell death.

Results {#Sec3}
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The data in Figure [1](#Fig1){ref-type=»fig»} show that conditioned media from hypoxic hMSCs are able to induce a hypoxia-like phenotype in undifferentiated hMSCs within 4 hours (Figure [1](#Fig1){ref-type=»fig»}) and 6 hours (Figure [2](#Fig2){ref-type=»fig»})

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Dec 20, 2018 · Movavi Video Converter Premium Crack [Paid Version] If you are a Movavi Vivida Premium subscriber, you can download Movavi.. Movavi Video Converter 19.2.4 Crack Activation Key Full Version.The present invention relates to a new and distinct cultivar of Pogostemon plant botanically known as Pogostemon cablin and hereinafter referred to by the cultivar name ‘Balbith’.
The new cultivar originated in a controlled breeding program in Messina-Sicilia, Italy during June 2012. The objective of the breeding program was the development of freely-flowering Pogostemon cultivars with numerous inflorescences and a well-branched, uniform and compact growth habit.
The new Pogostemon cultivar is the result of cross-pollination. The female (seed) parent of the new cultivar is the proprietary P. cablin breeding selection coded as ‘B8-13’, U.S. Plant Pat. No. 20,321, characterized by its dark pink-colored inflorescences, dark green-colored foliage, and moderately vigorous, compact-mounded growth habit. The male (pollen) parent of the new cultivar is the proprietary P. cablin breeding selection coded as ‘B-4-1’, U.S. Plant Pat. No. 23,689, characterized by its dark green-colored foliage, and moderately vigorous, compact growth habit. The new cultivar was discovered and selected as a single flowering plant within the progeny of the above stated cross-pollination during October 2013 in a controlled environment in Messina-Sicilia, Italy.
Asexual reproduction of the new cultivar by terminal stem cuttings since October 2013 in Messina-Sicilia, Italy and Elburn, III., USA has demonstrated that the new cultivar reprodu
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